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Journal of Veterinary Diagnostic Investigation, Vol 7, Issue 1, 98-101
Copyright © 1995 by American Association of Veterinary Laboratory Diagnosticians

Articles

Immunohistochemical detection of Tritrichomonas foetus in formalin-fixed, paraffin-embedded sections of bovine placenta and fetal lung

JC Rhyan, KL Wilson, DE Burgess, LL Stackhouse, and WJ Quinn

Pathobiology Laboratory, U.S. Department of Agriculture, Ames, IA 50010, USA.

An immunohistochemical technique using a monoclonal antibody was evaluated as a diagnostic tool to specifically label Tritrichomonas foetus in formalin-fixed, paraffin-embedded sections of placenta and fetal lung from bovine abortions. Trichomonads were demonstrated in tissues from each of 12 abortions due to T. foetus and none of 15 abortions due to other or unidentified causes. Moderate to marked background staining occurred only in severely autolyzed tissues from T. foetus-infected fetuses. The antibody faintly labeled 1 of 3 other species of trichomonads (Trichomonas gallinae) but did not label other protozoa, bacteria, or fungi tested.




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Copyright © 1995 by the American Association of Veterinary Laboratory Diagnosticians, Inc.