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Journal of Veterinary Diagnostic Investigation, Vol 6, Issue 3, 293-296
Copyright © 1994 by American Association of Veterinary Laboratory Diagnosticians

Articles

Cloning, expression, and sequence analysis of the ORF4 gene of the porcine reproductive and respiratory syndrome virus MN-1b

J Kwang, HS Kim, and HS Joo

USDA, ARS, US Meat Animal Research Center, Clay Center, NE 68933.

Porcine reproductive and respiratory syndrome virus (PRRSV) MN-1b strain open reading frame 4 (ORF4) has been cloned, sequenced, and expressed in Escherichia coli. The homologies of nucleotide and amino acid sequences between MN-1b (US isolate) and LV (European isolate) are 69% and 64%, respectively. The data also showed that ORF4 of MN-1b is 36 bases shorter than that of LV. Western blot analysis of expressed recombinant ORF4 protein reacted with 65% (26/40) of PRRSV-infected pig sera tested. These results demonstrated that ORF4 of PRRSV may not be a well-conserved region.




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Copyright © 1994 by the American Association of Veterinary Laboratory Diagnosticians, Inc.