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Journal of Veterinary Diagnostic Investigation, Vol 6, Issue 2, 153-155
Copyright © 1994 by American Association of Veterinary Laboratory Diagnosticians

Articles

Identification of African horse sickness virus in cell culture using a digoxigenin-labeled RNA probe

CC Brown, RF Meyer, and MJ Grubman

Foreign Animal Disease Diagnostic Laboratory, USDA, Greenport, NY 11944.

A digoxigenin-labeled RNA probe was synthesized from a plasmid containing a portion of the African horse sickness virus (AHSV) serotype 4 genome segment coding for nonstructural protein 1. In an in situ hybridization procedure, this probe hybridized successfully to Vero cells infected with each of the 9 serotypes of AHSV. There was no hybridization with noninfected cell cultures or cell cultures infected with bluetongue virus.




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Copyright © 1994 by the American Association of Veterinary Laboratory Diagnosticians, Inc.