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Journal of Veterinary Diagnostic Investigation, Vol 5, Issue 3, 368-371
Copyright © 1993 by American Association of Veterinary Laboratory Diagnosticians

Articles

Genus-specific detection of salmonellae using the polymerase chain reaction (PCR)

ND Cohen, HL Neibergs, ED McGruder, HW Whitford, RW Behle, PM Ray, and BM Hargis

Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.

Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.


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Rapid detection of food-borne pathogens by using molecular techniques
J. Med. Microbiol., January 1, 2005; 54(1): 51 - 54.
[Abstract] [Full Text] [PDF]


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