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Journal of Veterinary Diagnostic Investigation, Vol 5, Issue 3, 359-362
Copyright © 1993 by American Association of Veterinary Laboratory Diagnosticians

Articles

Development and evaluation of a mixed-antigen ELISA for serodiagnosis of Actinobacillus pleuropneumoniae serotypes 1, 5, and 7 infections in commercial swine herds

JT Bosse, R Friendship, S Rosendal, and BW Fenwick

Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.

A mixed-antigen enzyme-linked immunosorbent assay (ELISA) containing serotype-specific polysaccharide antigens from serotypes 1, 5, and 7 of Actinobacillus pleuropneumoniae was developed. This ELISA was evaluated using sera from experimentally infected pigs. With a negative cutoff value of 0.250 (optical density at 405 nm), sensitivity and specificity were determined to be 96% and 99.5%, respectively. The ELISA was further evaluated using sera from commercial swine. Sows and suckling piglets were found to be the best age groups for detection of positive reactors to A. pleuropneumoniae. The mixed-antigen ELISA could be used in a herd health monitoring program for detection of A. pleuropneumoniae infections.


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T. J. Inzana and B. Fenwick
Serologic Detection of Actinobacillus pleuropneumoniae in Swine by Capsular Polysaccharide-Biotin-Streptavidin Enzyme-Linked Immunosorbent Assay
J. Clin. Microbiol., April 1, 2001; 39(4): 1279 - 1282.
[Abstract] [Full Text]


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