JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rottinghaus, G.
Right arrow Articles by Hill, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rottinghaus, G.
Right arrow Articles by Hill, N.
Journal of Veterinary Diagnostic Investigation, Vol 5, Issue 2, 242-247
Copyright © 1993 by American Association of Veterinary Laboratory Diagnosticians

Articles

An HPLC method for the detection of ergot in ground and pelleted feeds

GE Rottinghaus, LM Schultz, PF Ross, and NS Hill

Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia 65211.

A high performance liquid chromatography (HPLC) method is described for detecting ergot in ground or pelleted forages and grains. Samples were extracted with alkaline chloroform, filtered, and applied to silica gel/organic binder cleanup columns. Following elution of pigments with acetone: chloroform, ergopeptine alkaloids were eluted with methanol and analyzed by HPLC with fluorescence detection. Average recovery of ergotamine, the major ergopeptine alkaloid produced by Claviceps, was 93%, with a relative standard deviation of 4.9%. The detection limit of ergotamine was approximately 50 ppb in all feedstuffs. Confirmation of ergopeptine alkaloids was accomplished by treating the parent ergopeptine alkaloids with 0.2% acetic acid to produce their -inine isomers and reexamining by HPLC with fluorescence detection or silica gel/organic binder column cleanup in combination with tandem mass spectroscopy. The method described is a valid alternative to microscopic inspection for detecting ergot contamination in ground or pelleted feedstuffs.


This article has been cited by other articles:


Home page
 Toxicol SciHome page
S. Bhusari, Z. Liu, L. B. Hearne, D. E. Spiers, W. R. Lamberson, and E. Antoniou
Expression Profiling of Heat Stress Effects on Mice Fed Ergot Alkaloids
Toxicol. Sci., January 1, 2007; 95(1): 89 - 97.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
R. S. Settivari, S. Bhusari, T. Evans, P. A. Eichen, L. B. Hearne, E. Antoniou, and D. E. Spiers
Genomic analysis of the impact of fescue toxicosis on hepatic function
J Anim Sci, May 1, 2006; 84(5): 1279 - 1294.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
D. E. Spiers, P. A. Eichen, and G. E. Rottinghaus
A model of fescue toxicosis: Responses of rats to intake of endophyte-infected tall fescue
J Anim Sci, June 1, 2005; 83(6): 1423 - 1434.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
B. G. Fieser and E. S. Vanzant
Interactions between supplement energy source and tall fescue hay maturity on forage utilization by beef steers
J Anim Sci, January 1, 2004; 82(1): 307 - 318.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association of Veterinary Laboratory Diagnosticians, Inc.