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Journal of Veterinary Diagnostic Investigation, Vol 5, Issue 2, 159-162
Copyright © 1993 by American Association of Veterinary Laboratory Diagnosticians

Articles

Use of a digoxigenin-labeled RNA probe to detect all 24 serotypes of bluetongue virus in cell culture

CC Brown, RF Meyer, and MJ Grubman

Foreign Animal Disease Diagnostic Laboratory, USDA, Greenport, NY 11944.

A digoxigenin-labeled RNA probe, corresponding to the section of the bluetongue virus (BTV) serotype 17 genome coding for nonstructural protein-1 (NS1), was applied to noninfected cell cultures and cell cultures infected with 24 different serotypes of BTV, 2 serotypes of epizootic hemorrhagic disease virus, and African horse sickness virus type 4. The probe hybridized to all cell cultures infected with the various BTV serotypes but did not hybridize to noninfected cell cultures or cell cultures infected with any of the other orbiviruses.


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S. J. Brodie, K. D. Bardsley, K. Diem, J. O. Mecham, S. E. Norelius, and W. C. Wilson
Epizootic Hemorrhagic Disease: Analysis of Tissues by Amplification and In Situ Hybridization Reveals Widespread Orbivirus Infection at Low Copy Numbers
J. Virol., May 1, 1998; 72(5): 3863 - 3871.
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Copyright © 1993 by the American Association of Veterinary Laboratory Diagnosticians, Inc.