JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collins, M.
Right arrow Articles by Whitlock, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collins, M.
Right arrow Articles by Whitlock, R.
Journal of Veterinary Diagnostic Investigation, Vol 5, Issue 1, 52-55
Copyright © 1993 by American Association of Veterinary Laboratory Diagnosticians

Articles

Reproducibility of a commercial enzyme-linked immunosorbent assay for bovine paratuberculosis among eight laboratories

MT Collins, A Angulo, CD Buergelt, SG Hennager, SK Hietala, RH Jacobson, DL Whipple, and RH Whitlock

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.

Interlaboratory reproducibility of an absorbed enzyme-linked immunosorbent assay (ELISA) kit for detection of bovine serum antibodies to Mycobacterium paratuberculosis was evaluated. A panel of 30 bovine sera (15 positives and 15 negatives) was tested in triplicate microtiter wells on each of 2 days at 8 different laboratories. One laboratory had invalid results because of positive or negative serum control optical density (OD) readings beyond the acceptable range specified by the kit. The coefficient of variation (CV) for mean OD values was influenced by low ODs on test negative sera at 2 laboratories, thus the CVs on positive sera were considered a more representative measure of kit reproducibility. Between-well CVs averaged 6.7% +/- 2.8% (mean +/- standard deviation), and between-day CVs averaged 14.5% +/- 9.8% among the 7 laboratories with valid assays on the 15 positive sera. The OD values were converted to positive or negative classifications for each assay well, and the results were compared. Among 1,392 assays in 7 laboratories, 98.6% were in agreement. Eleven of 18 discrepant results were due to a sample that consistently gave OD values near the cutoff for a positive test. Exclusion of that serum from the analysis resulted in a 99.8% rate of agreement among laboratories. Results indicated that the absorbed ELISA kit provided reproducible results within and between laboratories.


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
J. E. Lombard, B. A. Wagner, R. L. Smith, B. J. McCluskey, B. N. Harris, J. B. Payeur, F. B. Garry, and M. D. Salman
Evaluation of environmental sampling and culture to determine Mycobacterium avium subspecies paratuberculosis distribution and herd infection status on US dairy operations.
J Dairy Sci, November 1, 2006; 89(11): 4163 - 4171.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. P. Koets, V. P. M. G. Rutten, M. de Boer, D. Bakker, P. Valentin-Weigand, and W. van Eden
Differential Changes in Heat Shock Protein-, Lipoarabinomannan-, and Purified Protein Derivative-Specific Immunoglobulin G1 and G2 Isotype Responses during Bovine Mycobacterium avium subsp. paratuberculosis Infection
Infect. Immun., March 1, 2001; 69(3): 1492 - 1498.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association of Veterinary Laboratory Diagnosticians, Inc.