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Journal of Veterinary Diagnostic Investigation, Vol 4, Issue 4, 419-422
Copyright © 1992 by American Association of Veterinary Laboratory Diagnosticians

Articles

Use of ELISA to detect toxigenic Pasteurella multocida in atrophic rhinitis in swine

TL Bowersock, T Hooper, and R Pottenger

Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, W. Lafayette, IN 47907-1243.

The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermonecrotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates.


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R. L. Davies, R. MacCorquodale, S. Baillie, and B. Caffrey
Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis
J. Med. Microbiol., January 1, 2003; 52(1): 59 - 67.
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