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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 4, 463-471
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples

Kuo-Hua Lee, Jai-Wei Lee, Shih-Wen Wang, Lu-Yuan Liu, Mei-Fen Lee, Shih-Te Chuang, Yih-Min Shy, Chu-Li Chang, Ming-Che Wu and Chau-Hwa Chi1

Correspondence: 1Corresponding Author: Chau-Hwa Chi, Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei City, Taiwan 10617 ROC. chie{at}ntu.edu.tw

To efficiently prevent and treat bovine mastitis and minimize its effect on the dairy industry, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. In this study, a biochip capable of detecting 7 common species of mastitis-causing pathogens, including Corynebacterium bovis, Mycoplasma bovis, Staphylococcus aureus, and the Streptococcus spp. S. agalactiae, S. bovis, S. dysgalactiae, and S. uberis, within 6 hr was developed. The technique is based on DNA amplification of genes specific to the target pathogens and consists of 4 basic steps: DNA extraction of bacteria, polymerase chain reaction, DNA hybridization, and colorimetric reaction. To examine the accuracy and specificity of this biochip, a preliminary test with 82 random quarter milk samples were analyzed and compared with results from conventional microbiological methods conducted simultaneously. Results from all but 1 sample analyzed by the biochip were in agreement with those analyzed by bacteriology. The biochip could be a feasible tool for rapidly diagnosing mastitis-causing pathogens in milk and providing information for a more effective treatment to cure mastitis.

Key Words: Biochip • bovines • mastitis • pathogen






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