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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 3, 329-332
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communication

Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds

M. Paulina Fry, Juan Kruze1 and Michael T. Collins

Correspondence: 1Corresponding Author: Juan Kruze, Microbiology Department, Faculty of Sciences, Universidad Austral de Chile, PO Box 167, Valdivia, Chile, e-mail: jkruze{at}uach.cl

The accuracy of 4 commercial enzyme-linked immunosorbent assays (ELISAs) for diagnosis of bovine paratuberculosis was compared using sera from 53 Mycobacterium avium subsp. paratuberculosis (MAP) fecal culture–positive dairy cows (cases) and sera from 345 dairy cattle resident in 11 fecal culture–negative herds on 2 consecutive occasions 1 year apart (controls). The specificity of all 4 ELISA kits was >99%, and their diagnostic sensitivity ranged from 30.2% to 41.5%. Pairwise comparison of ELISAs found no significant differences (McNemar's chi-square test > 0.05), and assay agreement for categorical assay interpretation (positive or negative) was high (>98%) with {kappa} values ranging from 0.84 to 0.95. Receiver operating characteristic (ROC) curve analysis and the corresponding area under the ROC curves indicate that kit B had the highest overall accuracy. Thus, all 4 ELISA kits for bovine paratuberculosis had comparable accuracy when tested on Chilean dairy cattle, with kit B having a slight statistical advantage based on ROC area under the curve analysis. This suggests that any of the 4 kits could be appropriate for herd certification and for paratuberculosis control programs on Chilean dairy cattle.

Key Words: Bovines • diagnosis • enzyme-linked immunosorbent assay • Johne's disease • Mycobacterium paratuberculosis • Mycobacterium avium subsp. paratuberculosis • paratuberculosis






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