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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 1, 68-71
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communications

Molecular screening of canine GM1 gangliosidosis using blood smear specimens after prolonged storage: detection of carriers among shiba dogs in northern Japan

Osamu Yamato1, Eun-Og Jo, Hye-Sook Chang, Hiroyuki Satoh, Toru Shoda, Reeko Sato, Masami Uechi, Naomi Kawasaki, Yoshihisa Naito, Masahiro Yamasaki, Yoshimitsu Maede and Toshiro Arai

Correspondence: 1Corresponding Author: Osamu Yamato, Laboratory of Clinical Pathology, Department of Veterinary Clinical Sciences, Faculty of Agriculture, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan. osam{at}agri.kagoshima-u.ac.jp

Molecular screening of GM1 gangliosidosis in Shiba dogs was carried out in northern Japan using blood smear specimens after prolonged storage. Of 125 specimens obtained from 3 veterinary teaching hospitals for this screening, 68 specimens (54%) were adequate for direct amplification in a polymerase chain reaction (PCR)-based DNA test, and the percentage of adequacy was different at each hospital (34%, 73%, and 100%), suggesting that the amount of blood on the smear and the storage condition of specimens may affect adequacy. Of the 68 dogs examined, 2 dogs (2.9%) were heterozygous carriers for this disease and the other dogs were all genotypically normal. The results suggest blood smear specimens can be useful for PCR testing after prolonged storage provided specimens contain a generous amount of blood and have been adequately stored. The study also suggests that GM1 gangliosidosis may be widely prevalent in the Shiba dog population in northern Japan.

Key Words: Blood smear • GM1 gangliosidosis • Japan • molecular screening • Shiba dogs






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