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Comparison of retropharyngeal lymph node and obex region of the brainstem in detection of chronic wasting disease in white-tailed deer (Odocoileus virginianus)

Correspondence: ¹Corresponding Author: Wisconsin Veterinary Diagnostic Laboratory, University of Wisconsin, 445 Easterday Lane, Madison, WI 53706, e-mail: Delwyn.Keane{at}wvdl.wisc.edu

Chronic wasting disease (CWD) in Wisconsin was first identified in February 2002. By April 2005, medial retropharyngeal lymph node (RLN) tissues had been examined from over 75,000 white-tailed deer for the presence of CWD by either immunohistochemical (IHC) staining for the prion protein associated with CWD (PrP^res) or by using enzyme-linked immunosorbent assays with confirmation of positives by IHC staining and had been detected in 469 animals. Obex tissue was also available from 438 of the CWD-positive animals and was CWD positive by IHC staining in 355 (81%). To verify whether false-negative results were possible examining only RLN, both obex and RLN samples were examined for CWD by IHC staining from 4,430 of the white-tailed deer harvested from an area in Wisconsin where the overall deer CWD prevalence was approximately 6.2%. Two hundred and fourteen of the 269 positive deer (79.6%) had deposits of PrP^res in both obex and lymphoid tissues, 55 (20.4%) had deposits only in lymphoid tissue, and there were no deer that had deposits only in obex.

Key Words: Chronic wasting disease • enzyme-linked immunosorbent assay • immunohistochemistry • prion • white-tailed deer

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				D. P. Keane, D. J. Barr, P. N. Bochsler, S. M. Hall, T. Gidlewski, K. I. O'Rourke, T. R. Spraker, and M. D. Samuel Chronic wasting disease in a Wisconsin white-tailed deer farm J Vet Diagn Invest, September 1, 2008; 20(5): 698 - 703. [Abstract] [Full Text] [PDF]