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Journal of Veterinary Diagnostic Investigation, Vol 2, Issue 4, 334-337
Copyright © 1990 by American Association of Veterinary Laboratory Diagnosticians

Articles

Mycoplasma iowae species-specific DNA probe

SH Zhao and R Yamamoto

Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.

A Mycoplasma iowae (MI) species-specific DNA probe (designated pMI-2) of 6.0 kbp (kilobase pairs) was isolated from an MI strain I-695 genomic library prepared in plasmid pUC8 and Escherichia coli strain JM83. When labeled with [32]P by nick translation, the probe hybridized in dot blot assays with 6 reference strains and 8 field isolates of MI but not with 16 other known species of avian mycoplasmas. The pMI-2 probe detected a minimum of 1.5 ng of MI strain I-695 chromosomal DNA. Under identical conditions of hybridization, the probe did not hybridize with a high concentration (200 ng) of M. gallisepticum or M. synoviae chromosomal DNA.




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Copyright © 1990 by the American Association of Veterinary Laboratory Diagnosticians, Inc.