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Journal of Veterinary Diagnostic Investigation, Vol 2, Issue 3, 184-190
Copyright © 1990 by American Association of Veterinary Laboratory Diagnosticians

Articles

Comparison of polyacrylamide gel electrophoresis, an enzyme-linked-immunosorbent assay, and an agglutination test for the direct identification of bovine rotavirus from feces and coelectrophoresis of viral RNAs

S Hammami, AE Castro, and BI Osburn

Department of Veterinary Pathology, University of California-Davis 95616.

The dsRNA concentrated polyacrylamide gel electrophoresis (CPAGE) detected rotavirus directly from 19% of 77 stool specimens from diarrheic calves. A commercial enzyme-linked immunosorbent assay (ELISA) detected 25%, latex agglutination test, 23%, and polyacrylamide gel electrophoresis (PAGE), 19%. Establishing CPAGE as the "standard," the commercial ELISA and the latex agglutination test both had higher sensitivity (84%) than PAGE (79%). However, PAGE produced the highest specificity (100%), followed by agglutination (88%) and ELISA (84%). The commercial ELISA had a slightly higher sensitivity than agglutination, PAGE, and CPAGE, but the ELISA specificity was generally lower. The latex agglutination test had a lower sensitivity than ELISA, but specificity was higher. Agglutination had similar negative predictive values (94%), compared with agglutination and PAGe, but had the lowest positive predictive value (a measure of accuracy) (70%). Agreement with CPAGE was highest for PAGE (94.8%), followed by agglutination (87%) and ELISA (84.4%). The calculated percentages of total disagreement with all other tests indicated that ELISA differed from the other rotavirus detection assays in 10.4% of the cases, agglutination in 7.8%, PAGE in 2.6%, and CPAGE in 1.3%. The 2 PAGE assays allowed the detection of atypical rotaviruses from feces based on the characteristic "super-short" migration pattern of the 11 genomic segments of rotaviruses and of other members of the Reoviridae.


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R. K. Maes, D. L. Grooms, A. G. Wise, C. Han, V. Ciesicki, L. Hanson, M. L. Vickers, C. Kanitz, and R. Holland
Evaluation of a Human Group A Rotavirus Assay for On-Site Detection of Bovine Rotavirus
J. Clin. Microbiol., January 1, 2003; 41(1): 290 - 294.
[Abstract] [Full Text] [PDF]


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