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Journal of Veterinary Diagnostic Investigation, Vol 2, Issue 2, 132-134
Copyright © 1990 by American Association of Veterinary Laboratory Diagnosticians

Articles

A high-performance liquid chromatography method for determination of 4-aminopyridine in tissues and urine

SW Casteel and BR Thomas

Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia 65205.

An analytical method was developed to measure 4-aminopyridine in tissues and urine to determine appropriate diagnostic samples in acute poisoning cases. Tissues from rats dosed with 4-aminopyridine were extracted with methylene chloride. Extracts were analyzed by high-performance liquid chromatography using an isocratic solvent system of acetonitrile and aqueous solution (15/85 v/v) consisting of 0.015 M sodium salt of l-heptane-sulfonic acid, 0.002 M tetramethylammonium bromide, and 0.01 M sodium dihydrogen phosphate adjusted to pH 3.0 with phosphoric acid. We concluded that suitable diagnostic samples for acute poisoning cases include stomach contents, kidney, liver, and urine.




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