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Journal of Veterinary Diagnostic Investigation, Vol 2, Issue 2, 120-122
Copyright © 1990 by American Association of Veterinary Laboratory Diagnosticians

Articles

Identification of Brucella abortus strain 19 by decreased ability to utilize erythritol as determined by gas liquid chromatography

DR Ewalt, PF Ross, and JB Payeur

United States Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010.

A method to identify Brucella abortus strain 19 by erythritol utilization using gas liquid chromatography (GLC) was developed. A total of 69 strains of B. abortus (41 virulent field strain isolates and 28 strain 19 isolates) were tested. Following incubation of the isolate with a standard amount of erythritol, the erythritol present in the cell suspension was acetylated and measured by GLC. Field strains of B. abortus utilized an average of 90.9% of the erythritol, whereas vaccine strains utilized an average of 42.4%. This difference in erythritol utilization will allow a more rapid identification of B. abortus strain 19.




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Copyright © 1990 by the American Association of Veterinary Laboratory Diagnosticians, Inc.