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Journal of Veterinary Diagnostic Investigation, Vol 2, Issue 1, 24-28
Copyright © 1990 by American Association of Veterinary Laboratory Diagnosticians

Articles

The detection of antibodies to the glycoprotein X antigen of pseudorabies virus

D Cook, H Hill, M Snyder, P McMahon, and D Kinker

Veterinary Diagnostic Laboratory, Iowa State University, Ames 50010.

The persistence of antibodies to glycoprotein X (gpX) in the serum of pigs experimentally infected with pseudorabies virus (PRV) was determined using an anti-gpX enzyme-linked immunosorbent assay (ELISA). Antibodies to gpX were detected for at least 365 days postchallenge in nonvaccinated pigs. Previous sensitization of pigs by vaccination with S/PRV had no apparent effect on the antibody response of pigs to gpX postchallenge. In determining previous exposure of pigs to PRV strains containing the gpX gene, the anti-gpX ELISA was highly specific, but its sensitivity was lower than the standard serological procedures currently used for detecting PRV antibodies.




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Copyright © 1990 by the American Association of Veterinary Laboratory Diagnosticians, Inc.