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Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples

Correspondence: ¹Corresponding Author: Dr. Hugh Cai, Animal Health Laboratory, Laboratory Services Division, University of Guelph, PO Box 3612, Guelph, Ontario, Canada N1H 6R8, e-mail: hcai{at}lsd.uoguelph.ca

A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test–negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively.

Key Words: Bayesian modeling • enzootic pneumonia • FA test • Mycoplasma hyopneumoniae • PCR