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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 1, 103-106
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communications

Use of a polymerase chain reaction for detection of Mycoplasma dispar in the nasal mucus of calves

L.M. Marques, M. Buzinhani, M. Yamaguti, R.C. Oliveira, J.B. Ferreira, E. Mettifogo and J. Timenetsky1

Correspondence: 1Corresponding Author: J. Timenetsky, Instituto de Ciências Biomédicas II, Universidade de São Paulo, Rua Professor Lineu Prestes, 1374, CEP 05508 900, São Paulo, SP, Brazil

A polymerase chain reaction (PCR) assay was developed for the detection of Mycoplasma dispar in nasal mucus samples collected from calves. The target DNA sequence was the 16S rRNA gene, and the fragment was selected within a region of high polymorphism. The specificity and detection limit of the method were determined. This method was then used for the detection of M. dispar in nasal swabs collected from 301 calves, including 155 clinical samples from animals showing signs of respiratory disease and 146 samples from healthy animals. PCR with generic primers was applied to the detection of Mollicutes, followed by the detection of M. dispar. Mollicutes were detected in 52.05% of clinical samples from healthy animals and in 90.96% of samples from sick animals. Mycoplasma dispar was detected in 6.16% of healthy animals and in 34.84% of sick animals. The PCR assay was useful in verifying the presence of M. dispar in calves and may be a useful tool in monitoring this mycoplasma in cattle herds.

Key Words: Calves • Mycoplasma dispar • nasal mucus • PCR




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L. M. Marques, M. Buzinhani, R. C. Oliveira, M. Yamaguti, J. B. Ferreira, R. L. Neto, and J. Timenetsky
Prevalence of mycoplasmas in the respiratory tracts of calves in Brazil
Vet Rec., November 17, 2007; 161(20): 699 - 700.
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