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Journal of Veterinary Diagnostic Investigation Vol. 18 Issue 6, 545-552
Copyright © 2006 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Development and evaluation of an indirect enzyme-linked immunosorbent assay for detection of foot-and-mouth disease virus nonstructural protein antibody using a chemically synthesized 2B peptide as antigen

Toru Inoue, Satya Parida, David J. Paton, Wilai Linchongsubongkoch, David Mackay, Yooni Oh, Dilok Aunpomma, Simon Gubbins and Takakiyo Saeki1

Correspondence: 1Corresponding Author: Takakiyo Saeki, National Institute of Animal Health, Kodaira, Tokyo 187-0022, Japan

Forty peptides were synthesized corresponding to hydrophilic clusters of amino acids within the sequences of foot-and-mouth disease virus (FMDV) nonstructural proteins (NSP). Six peptides were studied in more detail and the most promising, a 2B peptide, was evaluated in enzyme-linked immunosorbent assay (ELISA) using sera from naïve, vaccinated, and vaccinated-and-challenged cattle as well as bovine sera from field outbreaks. The performance of the new NSP peptide ELISA was compared to that of 4 commercial NSP ELISA kits. Antibody to 2B was detectable from the end of the first week to the second week after infection in most of the nonvaccinated animals and by the second to third week in vaccinated-and-challenged animals. The sensitivity of the 2B peptide ELISA was comparable to the 3ABC Ceditest (Ceditest® FMDV-NS, Cedi Diagnostics B.V.; Chung et al., 2002). With some modification and further validation, this 2B test could be useful as a screening or conformational NSP test in postvaccination surveillance for FMD.

Key Words: 2B protein • foot-and-mouth disease virus • NSP ELISA • peptide antigens




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