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Journal of Veterinary Diagnostic Investigation, Vol 18, Issue 4, 398-401
Copyright © 2006 by American Association of Veterinary Laboratory Diagnosticians

Articles

First report of bluetongue virus serotype 1 isolated from a white-tailed deer in the United States

DJ Johnson, EN Ostlund, DE Stallknecht, VH Goekjian, M Jenkins-Moore, and SC Harris

Diagnostic Virology Laboratory, National Veterinary Services Laboratories, Ames, IA 50010, USA.

In November 2004, tissues were collected from a hunter-killed white-tailed deer in St. Mary Parish, Louisiana. Bluetongue virus (BTV) was isolated from the tissues; however, the isolate could not be identified as any of the US domestic serotypes. Subsequent testing by virus neutralization using serotype-specific antiserum tentatively identified the isolate as BTV serotype 1 (BTV-1), which had not previously been found in the United States. Primers were designed based on the sequence of an outer capsid protein gene of a South African BTV-1 strain. Reverse transcription-polymerase chain reaction testing with the BTV-1 primers and product sequencing confirmed the Louisiana isolate as BTV-1. This is the first report of BTV-1 in the United States.


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Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains
J. Gen. Virol., October 1, 2007; 88(10): 2811 - 2823.
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