JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McIntosh, K.
Right arrow Articles by Appleyard, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McIntosh, K.
Right arrow Articles by Appleyard, G.
Journal of Veterinary Diagnostic Investigation, Vol 18, Issue 4, 380-384
Copyright © 2006 by American Association of Veterinary Laboratory Diagnosticians

Articles

Nested polymerase chain reaction detection and duration of porcine circovirus type 2 in semen with sperm morphological analysis from naturally infected boars

KA McIntosh, JC Harding, S Parker, JA Ellis, and GD Appleyard

Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada.

A nested polymerase chain reaction (nPCR) protocol was applied to porcine semen to demonstrate the porcine circovirus type 2 (PCV2) shedding patterns and duration in naturally infected boars. Sperm morphology analysis was performed on a subset of samples to determine if the presence of PCV2 DNA in semen was associated with reduced semen quality. Semen was collected serially from 43 boars representing 6 breeds, aged 33.9 to 149.3 weeks. Of the 903 semen samples collected, 30 samples (3.3%) were positive for PCV2 DNA by nPCR from 13 boars. Boars shedding PCV2 DNA in semen ranged between 35.9 and 71.0 weeks of age, and shedding occurred during a period of up to 27.3 weeks. A semen nPCR test was 2.6 times more likely to be positive when collected from pigs that were < or =52 weeks of age, and 3.0 times more likely to be positive when collected from pigs that were < or =26 weeks from time of entry into the stud main unit (generalized estimating equations: P = 0.02; 95% confidence interval [CI] of the odds ratio 1.2 to 5.5, and P = 0.01; 95% CI of the odds ratio 1.3 to 6.9, respectively). These results demonstrate a sporadic and long-term shedding pattern of PCV2 DNA in semen from naturally infected boars. PCV2 DNA in semen does not appear to have detrimental effects on sperm morphology; however, boar age and, possibly, breed may contribute to the persistence of PCV2-shedding in semen.


This article has been cited by other articles:


Home page
 jvdiHome page
D. M. Madson, S. Ramamoorthy, C. Kuster, N. Pal, X.-J. Meng, P. G. Halbur, and T. Opriessnig
Characterization of shedding patterns of Porcine circovirus types 2a and 2b in experimentally inoculated mature boars
J Vet Diagn Invest, November 1, 2008; 20(6): 725 - 734.
[Abstract] [Full Text] [PDF]

Home page
 jvdiHome page
K. S. Lyoo, H. B. Kim, and H. S. Joo
Evaluation of a nested polymerase chain reaction assay to differentiate between two genotypes of Porcine circovirus-2
J Vet Diagn Invest, May 1, 2008; 20(3): 283 - 288.
[Abstract] [Full Text] [PDF]

Home page
 jvdiHome page
T. Opriessnig, X.-J. Meng, and P. G. Halbur
Porcine Circovirus Type 2 associated disease: Update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies
J Vet Diagn Invest, November 1, 2007; 19(6): 591 - 615.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Association of Veterinary Laboratory Diagnosticians, Inc.