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Journal of Veterinary Diagnostic Investigation, Vol 18, Issue 2, 215-217
Copyright © 2006 by American Association of Veterinary Laboratory Diagnosticians

Articles

Direct identification of Ehrlichia canis by a novel polymerase chain reaction method and molecular analysis of the citrate synthase (gltA) gene from various Italian strains

F Marsilio, B Di Martino, I Meridiani, and P Bianciardi

Department of Comparative Biomedical Sciences, University of Teramo, Italy. fmarsilio@unite.it

Fourteen blood samples collected from dogs that were seropositive for Ehrlichia canis were examined for the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. The assay detected E. canis DNA in 3 dogs. The complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from Italian isolates. The sequence data displayed high identity (99.2%) between the geographically separated Italian samples and the Oklahoma strain of E. canis. The high-sequence conservation revealed by molecular analysis confirmed the usefulness of the citrate synthase gene as a target for detection of E. canis.


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X. Zhang, T. Luo, A. Keysary, G. Baneth, S. Miyashiro, C. Strenger, T. Waner, and J. W. McBride
Genetic and Antigenic Diversities of Major Immunoreactive Proteins in Globally Distributed Ehrlichia canis Strains
Clin. Vaccine Immunol., July 1, 2008; 15(7): 1080 - 1088.
[Abstract] [Full Text] [PDF]


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