JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baszler, T.
Right arrow Articles by Hall, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baszler, T.
Right arrow Articles by Hall, S.
Journal of Veterinary Diagnostic Investigation, Vol 18, Issue 2, 147-155
Copyright © 2006 by American Association of Veterinary Laboratory Diagnosticians

Articles

Comparison of two automated immunohistochemical procedures for the diagnosis of scrapie in domestic sheep and chronic wasting disease in North American white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus)

TV Baszler, M Kiupel, ES Williams, BV Thomsen, T Gidlewski, DL Montgomery, KI O'Rourke, and SM Hall

Washington Animal Disease Diagnostic Laboratory and Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99165-20371, USA. baszlert@vetmed.wsu.edu

Two commercially available automated immunohistochemistry platforms, Ventana NexES and DakoCytomation Autostainer Universal Staining System, were compared for diagnosing sheep scrapie and cervid chronic wasting disease. Both automated platforms used the same antiprion protein monoclonal primary antibodies, but different platform-specific linker and amplification reagents and procedures. Duplicate sections of brainstem (at the level of the obex) and lymphoid tissue (retropharyngeal lymph node or tonsil) from the same tissue block were immunostained for the comparison. Examination of 1,020 tissues from 796 sheep revealed 100% concordance of results between the Ventana NexES and DakoCytomation platforms for diagnosing sheep scrapie from lymphoid tissue (103/103 positive; 405/405 negative) and brainstem (120/120 positive; 392/392 negative). Similarly, examination of 1,008 tissues from 504 white-tailed deer revealed 100% concordance between the Ventana NexES and DakoCytomation platforms for diagnosing chronic wasting disease from lymphoid tissue (104/104 positive; 400/400 negative) and brainstem (104/104 positive; 400/400 negative). Examination of 1,152 tissues from 482 mule deer revealed a concordance of 98.6% in lymphoid tissue and 99.9% in brainstem between the Ventana NexES and DakoCytomation platforms for diagnosing chronic wasting disease. The results indicate equivalence or near equivalence between the DakoCytomation and Ventana NexES autostainer platforms for identification of the disease-associated prion protein (PrPd)-positive and PrPd-negative brain and lymphoid tissues in sheep, white-tailed deer, and mule deer.


This article has been cited by other articles:


Home page
 jvdiHome page
J. A. Ramos-Vara, M. Kiupel, T. Baszler, L. Bliven, B. Brodersen, B. Chelack, S. Czub, F. D. Piero, S. Dial, E.J. Ehrhart, et al.
Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories
J Vet Diagn Invest, July 1, 2008; 20(4): 393 - 413.
[Abstract] [Full Text] [PDF]

Home page
 jvdiHome page
L. Manning, K. I. O'Rourke, D. P. Knowles, S. A. Marsh, Y. I. Spencer, E. Moffat, G. A.H. Wells, and S. Czub
A collaborative Canadian-United Kingdom evaluation of an immunohistochemistry protocol to diagnose bovine spongiform encephalopathy
J Vet Diagn Invest, July 1, 2008; 20(4): 504 - 508.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
M. M. Garner, K. Ramsell, N. Morera, C. Juan-Salles, J. Jimenez, M. Ardiaca, A. Montesinos, J. P. Teifke, C. V. Lohr, J. F. Evermann, et al.
Clinicopathologic Features of a Systemic Coronavirus-Associated Disease Resembling Feline Infectious Peritonitis in the Domestic Ferret (Mustela putorius)
Vet. Pathol., March 1, 2008; 45(2): 236 - 246.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Association of Veterinary Laboratory Diagnosticians, Inc.