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Journal of Veterinary Diagnostic Investigation, Vol 17, Issue 2, 198-200
Copyright © 2005 by American Association of Veterinary Laboratory Diagnosticians

Articles

Environmental air sampling to detect exotic Newcastle disease virus in two California commercial poultry flocks

SK Hietala, PJ Hullinger, BM Crossley, H Kinde, and AA Ardans

California Animal Health and Food Safety Laboratory, University of California, Davis, CA 95616, USA.

The 2002--2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.


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