Culture methods differ on the isolation of Salmonella enterica serotypes from naturally contaminated swine fecal samples

National Animal Disease Center, USDA, Ames, IA 50010, USA.

Four culture methods (A, B, C, and D) were comparatively evaluated for their ability to isolate Salmonella enterica from pooled swine fecal samples (n = 100). None of the methods was able to isolate Salmonella from all positive samples. The relative sensitivity of the culture methods evaluated was 82%, 94%, 95%, and 78% for methods A, B, C, and D, respectively. The comparison of sensitivities showed that methods B and C performed significantly better (P < 0.05) than methods A and D. Although relative sensitivities of methods B and C were equal, from the 89 positive samples concomitantly detected by both, 35 (39.3%) had different serotypes (no match) isolated by each method. On the basis of the results of this study, it was concluded that culture methods differ on the isolation of S. enterica serotypes from naturally contaminated swine fecal samples. Depending on the objective(s) of investigations on the ecology and epidemiology of S. enterica in swine populations, a method or a combination of methods should be considered for more reliable results.

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				B. C. Love and M. H. Rostagno Comparison of five culture methods for Salmonella isolation from swine fecal samples of known infection status J Vet Diagn Invest, September 1, 2008; 20(5): 620 - 624. [Abstract] [Full Text] [PDF]