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Journal of Veterinary Diagnostic Investigation, Vol 16, Issue 6, 503-508
Copyright © 2004 by American Association of Veterinary Laboratory Diagnosticians

Articles

Detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples: comparison of three polymerase chain reaction-based diagnostic tests with a conventional culture method

S Taddei, C Robbi, C Cesena, I Rossi, E Schiano, N Arrigoni, G Vicenzoni, and S Cavirani

Sezione di Malattie Infettive degli Animali, Dipartimento di Salute Animale, Universita di Parma, 43100 Parma, Italy.

Three commercially available assays, designed to specifically detect the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in fecal samples by IS900-PCR, were compared with a conventional culture method. Fecal samples from 100 dairy cows were tested. Fifty-four (67.5%) of 80 culture-positive samples were positive for an assay that detects MAP DNA by dot spot hybridization of polymerase chain reaction products (kit A), 48 (60%) were positive by an assay using ethidium bromide staining for agar gel visualization of amplification products (kit B), and 49 (61.3%) were positive by an assay in which amplified products are detected by a colorimetric detection system (kit C). Relative sensitivity of all tests increased in proportion to the presence of MAP in fecal samples. Specificity was 100% based on results from 20 culture-negative samples from an MAP-free herd.


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