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Journal of Veterinary Diagnostic Investigation, Vol 16, Issue 2, 164-166
Copyright © 2004 by American Association of Veterinary Laboratory Diagnosticians

Articles

Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2

I McNair, M Marshall, F McNeilly, A Botner, AS Ladekjaer-Mikkelsen, I Vincent, B Herrmann, R Sanchez, and C Rhodes

Department of Agriculture and Rural Development, Veterinary Sciences Division, Stormont, Belfast BT4 3SD, Northern Ireland.

A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assay (IPMA), and to determine the titer of each serum. Results from all centers were then compiled and correlated. They demonstrate a wide variation in the titers obtained between laboratories. These differences were dependent on the assay used and the choice of fixative. In general, IPMA gave higher titers than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus.


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T. Opriessnig, X.-J. Meng, and P. G. Halbur
Porcine Circovirus Type 2 associated disease: Update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies
J Vet Diagn Invest, November 1, 2007; 19(6): 591 - 615.
[Abstract] [Full Text] [PDF]


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