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Journal of Veterinary Diagnostic Investigation, Vol 12, Issue 6, 558-561
Copyright © 2000 by American Association of Veterinary Laboratory Diagnosticians


Articles

Polymerase chain reaction for definitive identification of Yersinia ruckeri

JT LeJeune and FR Rurangirwa

Department of Veterinary Microbiology and Pathology and the Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman 99164, USA.

Yersinia ruckeri causes enteric red mouth (ERM) disease in salmonids. Serologic identification of Y. ruckeri is hampered by cross-reactivity with other bacterial isolates of fish origin. Oligonucleotide primers incorporating Y. ruckeri unique sequences were designed to amplify a 409 bp fragment of Y. ruckeri 16S rDNA. The primers did not amplify other genetically related Yersinia or a wide variety of other aquatic or piscine bacteria. This assay provides a rapid, definitive identification of Y. ruckeri that is not subject to the variability inherent in serologic methods.





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