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Journal of Veterinary Diagnostic Investigation, Vol 12, Issue 3, 266-268
Copyright © 2000 by American Association of Veterinary Laboratory Diagnosticians

Articles

Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

MG Echeverria, EO Nosetto, and ME Etcheverrigaray

Area of Virology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.

A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions. A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.


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J. M. Laborde, C. Carbone, S. G. Corva, and C. M. Galosi
Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
J Vet Diagn Invest, November 1, 2008; 20(6): 789 - 791.
[Abstract] [Full Text] [PDF]


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