Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys

Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.

Antibodies to avian pneumovirus (APV) were first detected in Minnesota turkeys in 1997. Virus isolation was attempted on 32 samples (28 tracheal swabs, 4 pools of trachea and turbinates) that were positive for APV by reverse transcriptase polymerase chain reaction (RT-PCR). The cell cultures used were chicken embryo fibroblast (CEF), Vero cells, and QT-35 cells. Five virus isolates were obtained from these samples, and the identity of the isolates was confirmed by RT-PCR. Four isolates were obtained by inoculation of CEF cells, and 1 isolate was obtained in QT-35 cells after 3-7 blind passages in cell cultures. Vero cells did not yield any isolate on primary isolation; however, all 5 isolates could be adapted to grow in Vero cells following primary isolation in CEF or QT-35 cells. This is the first report of isolation of APV in Minnesota and also the first report of primary isolation of APV in QT-35 cells.

This article has been cited by other articles:

				C. Munoz-Zanzi, D. Trampel, T. Hanson, K. Harrison, S. Goyal, R. Cortinas, and D. Lauer Field estimation of the flock-level diagnostic specificity of an enzyme-linked immunosorbent assay for Avian metapneumovirus antibodies in turkeys J Vet Diagn Invest, March 1, 2009; 21(2): 240 - 243. [Abstract] [Full Text] [PDF]

				E. A. Turpin, D. C. Lauer, and D. E. Swayne Development and Evaluation of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Avian Metapneumovirus Type C-Specific Antibodies in Multiple Domestic Avian Species J. Clin. Microbiol., August 1, 2003; 41(8): 3579 - 3583. [Abstract] [Full Text] [PDF]

				A. M. Dar, S. Munir, S. M. Goyal, and V. Kapur Sequence Analysis of the Matrix (M2) Protein Gene of Avian Pneumovirus Recovered from Turkey Flocks in the United States J. Clin. Microbiol., June 1, 2003; 41(6): 2748 - 2751. [Abstract] [Full Text] [PDF]

				H.-J. Shin, K. T. Cameron, J. A. Jacobs, E. A. Turpin, D. A. Halvorson, S. M. Goyal, K. V. Nagaraja, M. C. Kumar, D. C. Lauer, B. S. Seal, et al. Molecular Epidemiology of Subgroup C Avian Pneumoviruses Isolated in the United States and Comparison with Subgroup A and B Viruses J. Clin. Microbiol., May 1, 2002; 40(5): 1687 - 1693. [Abstract] [Full Text] [PDF]

				F. F. Jirjis, S. L. Noll, D. A. Halvorson, K. V. Nagaraja, and D. P. Shaw Pathogenesis of Avian Pneumovirus Infection in Turkeys Veterinary Pathology, May 1, 2002; 39(3): 300 - 310. [Abstract] [Full Text] [PDF]

				B. R. Gulati, S. Munir, D. P. Patnayak, S. M. Goyal, and V. Kapur Detection of Antibodies to U.S. Isolates of Avian Pneumovirus by a Recombinant Nucleocapsid Protein-Based Sandwich Enzyme-Linked Immunosorbent Assay J. Clin. Microbiol., August 1, 2001; 39(8): 2967 - 2970. [Abstract] [Full Text] [PDF]