JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nussbaum, D.
Right arrow Articles by Rimmele, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nussbaum, D.
Right arrow Articles by Rimmele, D.
Journal of Veterinary Diagnostic Investigation, Vol 11, Issue 4, 314-318
Copyright © 1999 by American Association of Veterinary Laboratory Diagnosticians

Articles

Evaluation of quantitative latex agglutination for detection of Cryptosporidium parvum, E. coli K99, and rotavirus in calf feces

DJ Nussbaum, Salord JR, and DD Rimmele

Diagnostic Department, Vetoquinol S.A., Lure, France.

A new methodology for detection of rotavirus, Escherichia coli K99, and Cryptosporidium parvum in bovine fecal samples was developed based on a quantitative latex agglutination technique (QLAT). Calibrated microspheres coated with specific antibodies to 1 of the enteric pathogens are quantitatively agglutinated by the antigens present in diluted fecal sample. The test is performed in a 96-well flat-bottom plate. The samples were tested with a commercial enzyme-linked immunosorbent assay kit prior to being analyzed by QLAT. The calculated sensitivity and specificity are adequate for field conditions, because the amount of the pathogenic agents is generally high. The overall time to perform the test was about 20 minutes.


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
R. K. Maes, D. L. Grooms, A. G. Wise, C. Han, V. Ciesicki, L. Hanson, M. L. Vickers, C. Kanitz, and R. Holland
Evaluation of a Human Group A Rotavirus Assay for On-Site Detection of Bovine Rotavirus
J. Clin. Microbiol., January 1, 2003; 41(1): 290 - 294.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association of Veterinary Laboratory Diagnosticians, Inc.