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Journal of Veterinary Diagnostic Investigation, Vol 11, Issue 3, 215-220
Copyright © 1999 by American Association of Veterinary Laboratory Diagnosticians

Articles

Analysis of ruminant respiratory syncytial virus isolates by RNAse protection of the G glycoprotein transcripts

H Alansari, RB Duncan, JC Baker, and LN Potgieter

Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.

Two different respiratory syncytial virus (RSV) radiolabeled probes were used to characterize the genetic heterogeneity of 25 ruminant RSV isolates by the ribonuclease protection assay. A 32P-radiolabeled antisense RNA probe was transcribed from cloned ovine and bovine RSV G glycoprotein genes and then hybridized with total RNA isolated from infected cells with various ruminant RSV isolates. The results of this study, along with previously published nucleotide sequence data of the ovine RSV G glycoprotein gene, suggest the presence of at least 2 ruminant RSV subgroups. One subgroup is represented by RSV isolated from respiratory disease outbreaks from calves and goats, and the other is represented by RSV isolated from sheep.


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J. B. Rottman
The Ribonuclease Protection Assay: A Powerful Tool for the Veterinary Pathologist
Vet. Pathol., January 1, 2002; 39(1): 2 - 9.
[Abstract] [Full Text] [PDF]


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