JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lockhart, J.
Right arrow Articles by Davidson, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lockhart, J.
Right arrow Articles by Davidson, W.
Journal of Veterinary Diagnostic Investigation, Vol 11, Issue 1, 55-59
Copyright © 1999 by American Association of Veterinary Laboratory Diagnosticians

Articles

Evaluation of C3H/HeJ mice for xenodiagnosis of infection with Ehrlichia chaffeensis

JM Lockhart and WR Davidson

Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.

Because mice are experimentally susceptible to infection with Ehrlichia species, C3H/HeJ mice were evaluated as a potential xenodiagnostic model for detection of infection with and isolation of E. chaffeensis. Intraperitoneal inoculation of mice with E. chaffeensis-infected DH82 cell cultures produced seroconversion, with peak serum antibody titers of 1:256, at high dosages (>1.9 x 10(4) infected cells) but not at low dosages (1.9 or 1.9 x 10(2) infected cells). Ehrlichia chaffeensis was not reisolated from blood samples collected from inoculated mice on postinoculation day 21. Nested polymerase chain reaction (PCR), using primers specific for E. chaffeensis, was positive for only 2/70 (2.9%) tissue samples. A field evaluation in which C3H/HeJ mice were inoculated with blood and lymph node suspensions from 5 seropositive white-tailed deer, including 3 deer that were PCR positive for E. chaffeensis, failed to produce seroconversion in mice. The lack of seroconversion at low dosages, the failure to reisolate at any dosage, and the inability to confirm infection in PCR-positive field samples suggests C3H/HeJ mice are not a sensitive model for xenodiagnosis or detection of E. chaffeensis.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
J. R. C. delos Santos, K. Boughan, W. G. Bremer, B. Rizzo, J. J. Schaefer, Y. Rikihisa, G. R. Needham, L. A. Capitini, D. E. Anderson, M. Oglesbee, et al.
Experimental infection of dairy calves with Ehrlichia chaffeensis
J. Med. Microbiol., December 1, 2007; 56(12): 1660 - 1668.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
C. D. Paddock and J. E. Childs
Ehrlichia chaffeensis: a Prototypical Emerging Pathogen
Clin. Microbiol. Rev., January 1, 2003; 16(1): 37 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
E. A. Sotomayor, V. L. Popov, H.-M. Feng, D. H. Walker, and J. P. Olano
Animal Model of Fatal Human Monocytotropic Ehrlichiosis
Am. J. Pathol., February 1, 2001; 158(2): 757 - 769.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
V. G. Dugan, S. E. Little, D. E. Stallknecht, and A. D. Beall
Natural Infection of Domestic Goats with Ehrlichia chaffeensis
J. Clin. Microbiol., January 1, 2000; 38(1): 448 - 449.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association of Veterinary Laboratory Diagnosticians, Inc.