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Journal of Veterinary Diagnostic Investigation, Vol 1, Issue 4, 295-298
Copyright © 1989 by American Association of Veterinary Laboratory Diagnosticians

Case Reports

Canine pythiosis--isolation and identification of Pythium insidiosum

J Bentinck-Smith, AA Padhye, WR Maslin, C Hamilton, RK McDonald, and BJ Woody

College of Veterinary Medicine, Drawer V, Mississippi State University, MS 39762.

Pythium insidiosum was isolated from the subcutaneous tissue of a 1-year-old tan crossbreed dog and from the intestinal tract of an 18-month-old Samoyed male. Gomori's methenamine silver stain was superior to hematoxylin and eosin in demonstrating the organism in tissue sections. The agent was identified as P. insidiosum by zoospore formation in an aqueous yeast extract solution containing grass blades. Exoantigens produced in culture were shown to be identical to known P. insidiosum antigens by microimmunodiffusion.


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 MycologiaHome page
A. Schurko, L. Mendoza, A. W. A. M. de Cock, and G. R. Klassen
Evidence for geographic clusters: Molecular genetic differences among strains of Pythium insidiosum from Asia, Australia and the Americas are explored
Mycologia, March 1, 2003; 95(2): 200 - 208.
[Abstract] [Full Text] [PDF]


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